overexpression with pGEX

batu erman erman at brandeis.edu.bitnet
Fri Jun 18 19:29:00 EST 1993


Does anyone know whether the glutathione-s-transferase portion of a fusion 
protein created by cloning into the commonly available pGEX vectors binds
dimers? I am trying to see if my fusion protein is forming dimers or not,
and
I cannot cut my protein of interest away from the glutathione with Factor
Xa
or thrombin, and am working with the full protein to do my experiments
with.
Thanks,
Batu erman



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