louhelainen at louhelainen at
Fri Jun 18 08:03:21 EST 1993

In article <740348109snz at>, Duncan at ("Dr. Duncan Clark") writes:
> Hi Netters
> Instead of GeneClean I use agarase from NEB and just digest the agarose. It is
> very simple and I have used it for up to 10kb cloning without any problems.
> Hope this helps.
> Duncan
> -- 
> -----------------------------------------------------------------------------
>  Duncan Clark                       | Internet:   duncan at
>  GeneSys Ltd.                       | Compuserve: 100015.1406 at
> -----------------------------------------------------------------------------

  I have used the very same protocol. It's fast and it works.
  The only problems that I have had was when I tried to reduce
  the volume of the solution with isobutanol. I guess that I did'nt
  remove the oligosaccharides completely from the solution. 

  Beta-agarase is also nice in lambda/cosmid cloning, since the 
  inserts are very large and you have to avoid shearing of DNA.
(   Jari Pekka Louhelainen, MSc      "FastEddie"                               (
(   University of Helsinki, Dept. of General Microbiology                      (
(   Pl 41 (Mannerheiminitie 172), SF-00014 HELSINGIN YLIOPISTO,  FINLAND       (
(   Phone: +358-0-4735425,     Facsimile:	+358-0-4735426                 (

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