Geneclean

[louhelainen at cc.helsinki.fi]

In article <740348109snz at genesys.demon.co.uk>, Duncan at genesys.demon.co.uk ("Dr. Duncan Clark") writes:
> Hi Netters
> 
> Instead of GeneClean I use agarase from NEB and just digest the agarose. It is
> very simple and I have used it for up to 10kb cloning without any problems.
> 
> Hope this helps.
> 
> Duncan
> -- 
> -----------------------------------------------------------------------------
>  Duncan Clark                       | Internet:   duncan at genesys@demon.co.uk
>  GeneSys Ltd.                       | Compuserve: 100015.1406 at compuserve.com
> -----------------------------------------------------------------------------


  I have used the very same protocol. It's fast and it works.
  The only problems that I have had was when I tried to reduce
  the volume of the solution with isobutanol. I guess that I did'nt
  remove the oligosaccharides completely from the solution. 

  Beta-agarase is also nice in lambda/cosmid cloning, since the 
  inserts are very large and you have to avoid shearing of DNA.
  
 
  --
(   Jari Pekka Louhelainen, MSc      "FastEddie"                               (
(   University of Helsinki, Dept. of General Microbiology                      (
(   Pl 41 (Mannerheiminitie 172), SF-00014 HELSINGIN YLIOPISTO,  FINLAND       (
(   Phone: +358-0-4735425,     Facsimile:	+358-0-4735426                 (