RNase protection

[DG at dna.bio.warwick.ac.uk]

Dear Netters
Does anyone have any tips on increasing the sensitivity of RNase
protection analyses?  I am grateful for any suggestions on RNA
purification, probe synthesis, hybridization conditions, digestion
conditions, post-digestion treatments etc. etc. etc.
Thanks to all netters in advance
Derek Gatherer
University of Warwick - Biological Sciences