nash at biologysx.lan.nrc.ca
Fri Jun 18 10:42:43 EST 1993
In article <1993Jun18.152949.1 at vax1.tcd.ie> brennanp at vax1.tcd.ie writes:
>From: brennanp at vax1.tcd.ie
>Subject: Re: EtBr ussage
>Date: Fri, 18 Jun 1993 15:29:49 GMT
>I once heard an interesting suggestion that instead of soaking
>the gel in EtBr to add small amount of EtBr soln to the samples
>before running the gel. It still allows visualation but does
>not require the large amts of EtBr reqd for soaking gels.
>I have never used it myself but it sounded neat, maybe someone
>else knows more details
I only soak gels when I forget to add EtBr to my gel (or for PFGE, which
says not to). Add 15 ul of 4 mg/ml EtBr stock per 100 ml gel (worked out
empirically). Destain in tap water for 10 min.
When I soak gels, unless they are extensively washed, they leak EtBr... look
at your gloves under UV. EtBr pre-mixed in the gels never seems to leak
out, and it just a gel to dispose of, not litres of EtBr.
John Nash | Email: Nash at biologysx.lan.nrc.ca.
Institute for Biological Sciences |
National Research Council of Canada | Email to my other NRC accounts
Ottawa, Ontario, Canada. | is usually forwarded here.
*** Disclaimer: All opinions are mine, not NRC's! ***
More information about the Methods