Mitochondrial DNA isolation
wmelchior at ntet.nctr.fda.gov
Fri Jun 18 17:36:15 EST 1993
In article <1993Jun18.092202.5996 at gserv1.dl.ac.uk>,
CS at abc.univie.ac.at ("Christoph Schller") writes:
> Does anyone have a protocol to isolate mt-DNA from tissue (rat
> brain)? How to estimate nuclear DNA contamination? We want to analyse
> oxidative damage.
I've not tried it, but I have a copy of "A Rapid Procedure for Isolating
Mitochondrial DNA", E.G. Zimmerman et al, Gene Anal. Techn. 5:102-104
(1988). The example given is rat liver. The only "analysis" for
contamination by nuclear DNA seems to have been visual inspection of a gel.
The paper has several references to earlier methods.
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Bill Melchior ||
National Center for Toxicological Research || ALL statements
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