For Northern blots we add 1ul of a 1 mg/ml soln of EtBr to each sample just
prior to loading the gel. This greatly aids visualization of the RNA in the gel
without having to destain etc.
I have not tried this with DNA samples, but I can't see why it would not work.
St Jude Research Hospital
Elvis Land TN
In article <1993Jun18.152949.1 at vax1.tcd.ie>, brennanp at vax1.tcd.ie writes:
> On EtBr,
>> I once heard an interesting suggestion that instead of soaking
> the gel in EtBr to add small amount of EtBr soln to the samples
> before running the gel. It still allows visualation but does
> not require the large amts of EtBr reqd for soaking gels.
>> I have never used it myself but it sounded neat, maybe someone
> else knows more details
> Paul Brennan, Trinity College, Dublin, Ireland