Protein estimation in presence of detergents

Basavaraju Shankarappa bsh at MED.PITT.EDU
Mon Jun 21 10:37:13 EST 1993

Check out the BCA protein assay system from Pierce.  I have had very
good experience with this reagent.  It is compatible with upto
1% NP-40 (micro version), 1% SDS etc.,.
Raj Shankarappa
bsh at
> I am looking for a method for determination of protein which is
> not affected by detergents such as Triton X100 or SDS.  I understand
> that there is a modification of the Coomassie Blue dye binding
> originally assay published by Marion Bradford (Anal Biochem, 1976) which is
> is insensitive to SDS and other detergents.  However I can't find
> the reference.  Can anyone help with this method or any other?
> Dr Connor J Thomas
> Department of Microbiology and Immunology
> University of Adelaide
> Adelaide, Australia
> Email: cthomas at
> -------------------------------------

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