Sequenase on the 373

Rick Wilson rick at GENEMAN.WUSTL.EDU
Mon Jun 21 07:33:28 EST 1993


Sorry about the two truncated postings.

What I was going to say was that wetsel_r at msgcg.wustl.edu had a question about
using Sequenase on the ABI sequencer.  Bruce Roe correctly replied that to use
Sequenase and dye-terminators, one needs to upgrade the sequencer with a five-
color filter wheel.  However, if you want to use Sequenase with dye-primers,
no upgrade is necessary.  You'll want to make sure you've got the kind of
template DNA that looks good with isotopic sequencing, but more of it (e.g.
2 ug of M13).

R. Wilson
St. Louis




More information about the Methods mailing list