adleman at pollux.usc.edu
Mon Jun 21 23:24:01 EST 1993
I hope that this is not too stupid a question, I am new at this.
I would like to induce the expression of a short peptide (8 amino
acids) in E. coli. I intended to use pUC18. How can I avoid making a
fusion protein where my peptide is proceeded by Thr Met Ile Thr Asn
Ser (which are encoded by the base pairs preceding the polylinker)?
Is there a simple approach? For example, can I modify my
oligonucleotide by giving it its own promoter?
Professor Len Adleman
University of Southern California
Adleman at pollux.usc.edu
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