pUC18 question

Len Adleman adleman at pollux.usc.edu
Mon Jun 21 23:24:01 EST 1993


Dear Researchers,

I hope that this is not too stupid a question, I am new at this.

I would like to induce the expression of a short peptide (8 amino
acids) in E. coli. I intended to use pUC18. How can I avoid making a
fusion protein where my peptide is proceeded by Thr Met Ile Thr Asn
Ser (which are encoded by the base pairs preceding the polylinker)?

Is there a simple approach?  For example, can I modify my
oligonucleotide by giving it its own promoter?


Thanks
Professor Len Adleman
University of Southern California
Adleman at pollux.usc.edu



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