in vitro transcriptions

Mark Henteleff markh at ahi.pbrc.hawaii.edu
Tue Jun 22 17:09:50 EST 1993


I am currently doing in vitro transcription protocols to make
mRNA for Na channel (approx. 6kb) to use in expression in
Xenopus oocytes.  Anybody out there know if its necessary
to get rid of unincorporated nucleotides before the sample can
be used in these kinds of expression experiments?

Thanks   Mark H.



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