Suggestions needed for PCR

[Paul N Hengen]

In article <MAILQUEUE-101.930622100350.399 at micro.uct.ac.za> ed at micro.uct.ac.za writes:
 
>> can anybody tell me what "variables" can be modified that can possibly
>> improve amplification (ie. changing the amounts of certain reagents; adding
>> "catalytic" reagents; changing the number of cycles).... 

>These and other parameters are ably and amply discussed in:
>
>Innis MA and Gelfand DH (1990).  Optimization of PCRs.  pp. 3-12 in: "PCR
>    Protocols (Innis, Gelfand, Sninsky and White, eds.); Academic Press,
>    New York.

I think it would do you a world of good to read the PCR references listed
in the FAQ list. If you haven't seen it yet, I can send you a copy by e-mail.

Paul N. Hengen
National Cancer Institute
Frederick Cancer Research and Development Center
Frederick, Maryland 21702-1201 USA