DNA Contamination in PCRs

Tue Jun 22 19:35:53 EST 1993

Hello everyone,

Another lab was describing problems they have encountered in PCR where their
negative controls are giving positive signals -- contaminated reagents!  I
suggested that they aliquot all fresh reagents and never use the same source
twice.  In addition, they have also been using plugged pipette tips.  I think  
this should work well, but they were wondering how reagents could be treated   
(apart from autoclaving) that may eliminate contamination.  They have already  
spent a fair amount of money on new reagents for RT-PCR and would like to know 
if they could use some method such as UV irradiation, etc.  Does anyone know
any specific procedures?

Thanks in advance for any suggestions you might have.


Patrick Farley
farleyp at HAL.Hahnemann.edu

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