mRNA extraction from tissue options ...
steven joseph chmura
sjchmura at kimbark.uchicago.edu
Wed Jun 23 14:52:49 EST 1993
Has anyone used any really good kits for obtainting clean mRNA from tissue?
I am trying to get mRNA out of gut and while I have very good OD readings
(>1.9) there is definite genomic DNA contaminants. I cannot use exon
trapping for my PCR (I must use one exon due to homologies).
Anyway, I need to get rid of the DNA and keep the RNA. There is no
100% RNAse free DNAse so I have already thrown that idea out.
Anyone had any luck and no DNA>>>????
Thanks a lot as always....
________________________________________________________________________________Steven Chmura University of Chicago Medical School(M1)
"Given enough time, the impossible becomes probable, and the probable
inevitable.." -George Wald, "On the Origins of Life"
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