Address for PCR methods and applications!!!
bsh at MED.PITT.EDU
Wed Jun 23 08:30:15 EST 1993
As to an important aspect, I think all the other journals should follow
the BioTechniques' lead in providing email addresses of the authors in
> I'm trying to find out a copy of the journal "PCR methods and applications"?
> to identify the publisher. I heard its Cold Spring Harbor Press but I'm not
> sure. If some one has access to this journal I will appreciate if you can tell
> me the address to request a copy from the publisher or subscription
Cold Spring Harbor Laboratory Press
1 Bungtown Raod
Cold Spring Harbor
Phone: (516) 367-8492
Fax: (516) 367-8532
bsh at med.pitt.edu
> A. Alvarez
> alvarez at mbcf.stjude.org
> In article <1993Jun17.102159.2504 at gnv.ifas.ufl.edu>, afc at gnv.ifas.ufl.edu writes:
> > In article <1993Jun15.145544.21902 at gserv1.dl.ac.uk>, CS at abc.univie.ac.at ("Christoph Schller") writes:
> >> Hi
> >> We frequently (with different batches enzyme) observe the
> >> disappearing of PCR products if they are EtOH prec. O/N on -20.
> >> Has anybody else observed that? - And how to get rid of this
> >> mysterious nuclease (phenol doesn't work).
> >> Thanks
> >> Christoph S.
> > IMHO, your problem is with precipitation, not nucleases. No enzyme is
> > going to degrade ethanol precipitated nucleic acid.
> > To begin with, precipitate your DNA at +4C, not -20C. It is well
> > known (except by my graduate students) that DNA precipitates much more
> > effectively at the higher temperature.
> > Second, centrifuge the ethanol precipitate at a higher speed or for a longer
> > time. This is important if you have a relatively small amount of product.
> > 30 minutes at 12,000 RPM is probably good.
> > Finally, add some carrier, either nucleic acid or protein.
> > Andrew Cockburn
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