Protein estimation in presence of detergents
mligr at flash.LakeheadU.Ca
Wed Jun 23 20:07:38 EST 1993
cthomas at science.adelaide.edu.au writes:
>I am looking for a method for determination of protein which is
>not affected by detergents such as Triton X100 or SDS. I understand
>that there is a modification of the Coomassie Blue dye binding
>originally assay published by Marion Bradford (Anal Biochem, 1976) which is
>is insensitive to SDS and other detergents. However I can't find
>the reference. Can anyone help with this method or any other?
>Dr Connor J Thomas
>Department of Microbiology and Immunology
>University of Adelaide
>Email: cthomas at microb.adelaide.edu.au
I've just came accross 2 articles that might be interesting for you:
Horikawa,S.;Ogawara,H. (1979): A simple and rapid procedure for removal of
Triton X-100 from protein solution. Anal biochem 97(1):116-119.
Mather,I.A.;Tamplin,C.B. (1979): A method for the determination of protein in
the presence of Triton X-100. Anal Biochem 93(1):139-142.
These two work just with non-ionic detergents. If you find some simple way
how to get rid of the SDS, please let me know.
Martin Ligr < mligr at flash.lakeheadu.ca >
Thunder Bay, Ontario
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