Protein estimation in presence of detergents

[Martin Ligr]

cthomas at science.adelaide.edu.au writes:

>Hi,

>I am looking for a method for determination of protein which is
>not affected by detergents such as Triton X100 or SDS.  I understand
>that there is a modification of the Coomassie Blue dye binding
>originally assay published by Marion Bradford (Anal Biochem, 1976) which is
>is insensitive to SDS and other detergents.  However I can't find
>the reference.  Can anyone help with this method or any other?

>Dr Connor J Thomas
>Department of Microbiology and Immunology
>University of Adelaide
>Adelaide, Australia
>Email: cthomas at microb.adelaide.edu.au

I've just came accross 2 articles that might be interesting for you:
Horikawa,S.;Ogawara,H. (1979): A simple and rapid procedure for removal of
Triton X-100 from protein solution. Anal biochem 97(1):116-119.
Mather,I.A.;Tamplin,C.B. (1979): A method for the determination of protein in
the presence of Triton X-100. Anal Biochem 93(1):139-142.

These two work just with non-ionic detergents. If you find some simple way
how to get rid of the SDS, please let me know.

Bye.

Martin Ligr < mligr at flash.lakeheadu.ca >
Thunder Bay, Ontario