DNA Contamination in PCRs
Vmiao at oregon.uoregon.edu
Wed Jun 23 19:18:48 EST 1993
In article <0096E6BC.E8B032E0.27546 at hal.hahnemann.edu>,
farleyp at HAL.HAHNEMANN.EDU wrote:
> Another lab was describing problems they have encountered in PCR where their
> negative controls are giving positive signals -- contaminated reagents!
They have already
> spent a fair amount of money on new reagents for RT-PCR and would like to know
> if they could use some method such as UV irradiation, etc. Does anyone know
> any specific procedures?
I don't recall a specific procedure, but I think that Stratagene once wrote
up about using their UV crosslinker to decontaminant PCR reagents. Maybe
colleagues could give the company a call to get some details.
More information about the Methods