DNA Contamination in PCRs

Vivian Miao Vmiao at oregon.uoregon.edu
Wed Jun 23 19:18:48 EST 1993


In article <0096E6BC.E8B032E0.27546 at hal.hahnemann.edu>,
farleyp at HAL.HAHNEMANN.EDU wrote:
> 
> Another lab was describing problems they have encountered in PCR where their
> negative controls are giving positive signals -- contaminated reagents! 
 (stuff deleted)
They have already  
> spent a fair amount of money on new reagents for RT-PCR and would like to know 
> if they could use some method such as UV irradiation, etc.  Does anyone know
> any specific procedures?

I don't recall a specific procedure, but I think that Stratagene once wrote
something
up about using their UV crosslinker to decontaminant PCR reagents.  Maybe
your
colleagues could give the company a call to get some details. 



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