EtBr Usage

Bob DeLisio delisior at rnisd0.DNET.roche.com
Thu Jun 24 18:29:55 EST 1993


Hello All:

Someone in this thread suggested putting the microwave in the hood when
melting agarose that contains etbr. In my lab we do not allow solutions
with etbr anywhere near the microwave!

We have found that if you double the agarose concentration of the bottle you 
are heating and omit the etbr you can then cool it down with an equal volume of 
a solution containing 2X etbr in 1X running buffer. This avoids volatilizing 
etbr and helps speed the gel solidification.

Regards
Bob



=====================================================
 _/   _/     Robert DeLisio
  _/ _/      Roche Institute of Molecular Biology
    _/       Roche Research Center
   _/ _/     Nutley, New Jersey 07110-1199
  _/   _/
   _/ _/     Voice: (201) 235-3728
     _/      Fax:   (201) 235-2839
    _/ _/    Internet: delisior at rnisd0.dnet.roche.com
   _/   _/   CompuServe: 73517,2616
=====================================================




More information about the Methods mailing list