DNA Contamination in PCRs

Terese M Barta-1 barta001 at STAFF.TC.UMN.EDU
Thu Jun 24 17:26:33 EST 1993



On 24 Jun 1993, Vivian Miao wrote:

> > Another lab was describing problems they have encountered in PCR where
their
> > negative controls are giving positive signals -- contaminated reagents! 
>  (stuff deleted)
> They have already  
> > spent a fair amount of money on new reagents for RT-PCR and would like to know 
> > if they could use some method such as UV irradiation, etc.  Does anyone know
> > any specific procedures?
> 
A method for UV-treating PCR rxns to eliminant DNA contaminants can be
found in Nucleic acids research 18: 6673-6676 (1990, Rich and Willis).

T. Barta
GFBI
University of Minnesota




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