inclusion body mutant

Daniel Mytelka mytelka at zenith.berkeley.edu
Wed Jun 23 23:19:22 EST 1993


>In article <1993Jun23.162856.9130 at infodev.cam.ac.uk> pra1 at mbuf.bio.cam.ac.uk writes:
>
>>  Does anyone know of a strain of E. coli that does not form inclusion 
>>bodies?                             
>>



I don't know of any strains that don't produce inclusion bodies, but
two genes have recently been identified whose products are intimately
associated with inclusion bodies and which are overproduced in response
to expression of heterologous proteins. It's possible that knocking
one or both of these (homologous) genes out would lead to fewer or
no inclusion bodies. The authors may even have tried this by now, so
you can try contacting them.

Reference: J Bac (Nov 1992) 174(21): 6938-6947.

Good Luck,

 Daniel Mytelka (mytelka at mendel.berkeley.edu)



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