al18 at cornell.edu
Fri Jun 25 10:48:38 EST 1993
I'm doing methylation interference experiments on double stranded
oligonucleotides and am having problems with what looks like a background
cleavage at non specific bases. Thus, I am getting cleavage at bases
other than G and As and therefore I cannot obtain a clear interference
pattern. Troubleshooting so far:
1. It is not a nuclease problem - without DMS there is no cleavage
2. The oligo is fine as after purification the oligonucleotide is a
single band on a denaturing gel
3. This oligonucleotide sequence has an inosine residue in it and I
don't know if this background is due to this residue.
Please help me with this problem.
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