F plasmid won't transfer

Robert Preston rapr at MED.PITT.EDU
Sat Jun 26 12:14:54 EST 1993


Jim Graham writes:
> 
> After "rescuing" the chlormaphenicol marked F plasmid from CJ236 by matings
> to the ung- mutant BW310, I cannot get the plasmid to transfer to a 
> third strain, MG4. MG4 is a "wild type" save for deletion in the lac operon
> and a recA mutation. That is BW310F'(cmR) matings with MG4 fail to transfer the
> marked F plasmid. 
> 
> Any ideas ?

How about growing the MG4 on ethanol-supplemented LB?  (can't remember
the rest of the recipe that went,"...candy is dandy but likker is quicker"
........Rob

Robert A. Preston
rapr at med.pitt.edu
> 



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