F plasmid won't transfer

the End jgraham at bronze.ucs.indiana.edu
Sat Jun 26 08:13:37 EST 1993


After "rescuing" the chlormaphenicol marked F plasmid from CJ236 by matings
to the ung- mutant BW310, I cannot get the plasmid to transfer to a 
third strain, MG4. MG4 is a "wild type" save for deletion in the lac operon
and a recA mutation. That is BW310F'(cmR) matings with MG4 fail to transfer the
marked F plasmid. 

Any ideas ?

Jim
J. Graham
.



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