Gel leaks (was Salt Gradient Gels)
zxmkr08 at studserv.zdv.uni-tuebingen.de
Sun Jun 27 12:31:29 EST 1993
We don't seal our sequence gels at all. We use a Pharmacia
apparatus to cast 0.2 mm thin gels, but it should be possible
to do without it. And this is the way it works:
1) Lay one of the two plates perfectly flat. The plate should
not be able to slide around (Pharmacia uses rubber stripes
to prevent this).
2) Clamp the left and right spacers to this plate. There must
be enough place for the other plate to fit inbetween the
3) Lay the other plate on the bottom of the first one. Elevate
the top of the first plate a bit.
4) Now pour the acrylamide mix just before the top of the
second plate while pushing it gently towards the top of
the first. Avoid capturing air bubbles.
5) When you have arrived at the top of the first plate, there
is usually air between the lower parts of the plates. You
get this air out by elevating the top of the plates and
pouring some acrylamide on there. The acrylamide flows
down between the plates and pushes the air out.
6) Clamp the upper plate to the lower one, insert comb and
let the gel polymerize.
I hope that one can guess the way it works from my attempts
to describe it. Pouring the gels is much easier :-)
/* Cornelius Krasel, Department of Physiological Chemistry, U Tuebingen */
/* email: krasel at studserv.zdv.uni-tuebingen.de */
/* "People are DNA's way of making more DNA." (R. Dawkins / anonymous) */
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