CJ236 F' episome ?

the End jgraham at bronze.ucs.indiana.edu
Mon Jun 28 10:15:38 EST 1993


Can anyone support the rumor that there are genes necessary for transfer
of the cm marked F plasmid found in CJ236 on an additional pCYC based 
episome ? The cm marker seems to transfer well when CJ236 is mated with
a ung (uracil glycosylase) mutant, but the recipient does not appear to
be able to pass the marker on in matings to a third strain. The literature
on the background of this strain is relatively sketchy.

Any information on the construction of CJ236 greatly appreciated.

Thanks,

Jim
J. Graham
.



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