cheap spin columns

Andy Dizon andy at POOH.UCSD.EDU
Mon Jun 28 11:30:58 EST 1993


In article <1993Jun25.230351.1370 at Princeton.EDU>, 
lpcasson at phoenix.Princeton.EDU (Lawrence P. Casson) writes:
> I'm looking for an inexpensive way to purify the products of a
> cycle-sequencing reaction from dye-terminators (from ABI sequencing
> kit).  As I need to sequence a hundred or so mutants of the same
> sequence, I wonder if the purification can be done for much less than
> $3.00 per reaction (the cost of spin columns from Princeton Separations
> which work quite well for me - I don't know what's in them).
                                                                        
We re-fill our Princeton Separations columns with an equivalent amount of
Sephadex G50 fine.  We just knock old the old sepadex, let them dry, and
reload them.

Andrew
Southwest Fisheries Science Center
PO Box 271
La Jolla CA 92038



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