Isolation of asitic lymphocites

[Raul Arredondo]

   Dear Colleagues:
   We would greatly appreciate if somebody out there could
advice us in regard on protocols for lymphocite isolation from
asitic liquid. In our lab, we routinely isolate white cells 
from blood using Ficoll gradients, however this is non
practical for asitic large volumes (7 L). Does anybody know
alternative procedures?
Thank you very much in advance.
Raul Arredondo-Peter
<RARREDON at IFCSUN1.IFISIOL.UNAM.MX>