CJ236 F' episome ?

Dr. Duncan Clark Duncan at genesys.demon.co.uk
Mon Jun 28 15:40:34 EST 1993


In article <C9C7q2.n0J at usenet.ucs.indiana.edu> jgraham at bronze.ucs.indiana.edu writes:

>Can anyone support the rumor that there are genes necessary for transfer
>of the cm marked F plasmid found in CJ236 on an additional pCYC based 
>episome ? The cm marker seems to transfer well when CJ236 is mated with
>a ung (uracil glycosylase) mutant, but the recipient does not appear to
>be able to pass the marker on in matings to a third strain. The literature
>on the background of this strain is relatively sketchy.
>
>Any information on the construction of CJ236 greatly appreciated.
>
If I remember correctly, CJ236 was made by Catherine Joyce of Klenow clone
fame. Just find any paper on Klenow mutants and you will be able to track her
down.

Could the non-transfer be due to hsd restriction? 

Duncan
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