CJ236 F' episome ?

Dr. Duncan Clark Duncan at genesys.demon.co.uk
Mon Jun 28 15:40:34 EST 1993

In article <C9C7q2.n0J at usenet.ucs.indiana.edu> jgraham at bronze.ucs.indiana.edu writes:

>Can anyone support the rumor that there are genes necessary for transfer
>of the cm marked F plasmid found in CJ236 on an additional pCYC based 
>episome ? The cm marker seems to transfer well when CJ236 is mated with
>a ung (uracil glycosylase) mutant, but the recipient does not appear to
>be able to pass the marker on in matings to a third strain. The literature
>on the background of this strain is relatively sketchy.
>Any information on the construction of CJ236 greatly appreciated.
If I remember correctly, CJ236 was made by Catherine Joyce of Klenow clone
fame. Just find any paper on Klenow mutants and you will be able to track her

Could the non-transfer be due to hsd restriction? 

 Duncan Clark                       | Internet:   duncan at genesys@demon.co.uk
 GeneSys Ltd.                       | Compuserve: 100015.1406 at compuserve.com

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