Problems with Promega silver sequencing kit

[Claus Jorgensen]

In article <1993Jun23.200801.6172 at news.yale.edu> YU at biomed.med.yale.edu writes:

>We tried the Promega silver sequencing kit. We have problems with it
>The biggest being partof the gel stuck to the siliconized plate instead
>of the "bind silane"-treated plate and thus splitting the gel. Anybody
>has any ideas/similar expierences?
>Thanks for your time.
>
Indeed, I have had the same problem. But since I have started to treat both 
plates the day before I pour the gel, the problem have disappeared. I think 
the reason for my problem was that the silane plate didn't manage to dry 
before I poured the gel.

I hope this helps


Regards,

Claus Jorgensen
Dept. of Immunolgy
AFRC Babraham Institute
Cambridge CB2 4AT, UK.