Problems with Promega silver sequencing kit
claus at tamarin.demon.co.uk
Wed Jun 30 13:16:27 EST 1993
In article <1993Jun23.200801.6172 at news.yale.edu> YU at biomed.med.yale.edu writes:
>We tried the Promega silver sequencing kit. We have problems with it
>The biggest being partof the gel stuck to the siliconized plate instead
>of the "bind silane"-treated plate and thus splitting the gel. Anybody
>has any ideas/similar expierences?
>Thanks for your time.
Indeed, I have had the same problem. But since I have started to treat both
plates the day before I pour the gel, the problem have disappeared. I think
the reason for my problem was that the silane plate didn't manage to dry
before I poured the gel.
I hope this helps
Dept. of Immunolgy
AFRC Babraham Institute
Cambridge CB2 4AT, UK.
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