Methods June 1993 Archives by thread
Starting: Tue Jun 1 03:49:00 EST 1993
Ending: Wed Jun 30 23:38:29 EST 1993
Messages: 526
- CAT assay reproducability-response
GRGGTA at PATERSON.CHRISTIE-HOSPITAL.MANCHESTER.AC.UK
- CAT assays reproducability - responses
GRGGTA at PATERSON.CHRISTIE-HOSPITAL.MANCHESTER.AC.UK
- epitope size
Pierre Martineau
- Request for software for matrix construction
Raul Arredondo
- unsubscribe
angus at CATS.UCSC.EDU
- Cross banding on Westerns using monoclonal Ab? help!
Mr. A.F. Hill
- Problems with Promega Taq
Patricia Foster
- Techne / Stratagene thermal cyclers
Ed Rybicki
- Help:Cyanobacteria
botjlh at lure.latrobe.edu.au
- Information about pBIN19
u6069416 at ucsvc.ucs.unimelb.edu.au
- Windows DotPlot
Ramin Charles Nakisaramin
- Encyclopedia of the Mouse Genome Now available by Gopher
Dan Jacobson
- phagemids vs plasmids
isbruck at ac.dal.ca
- Multi-pipettors and DNA sequencing
hey at mbcl.rutgers.edu
- is it possible to just cut the 1-2 base mismatched hairpin?
Basavaraju Shankarappa
- Western Blotting with TransaBind
Anthony Shearing
- blue plastic pestle
Ding Ming
- Blue pestle
C.
- Western Blotting with TransaBind
Ed Rybicki
- re cycle sequencing
skspoidn at reading.ac.uk
- cDNAs & ORFs
Andre Hamel
- Xpress system - enterokinase and antibodies
SCHULZ
- RECOMBINANT PLASMID wanted: see inisde... PLEASE, HELP
Marina V Knyazeva
- Western Blotting with TransaBind
Anthony Shearing
- help!!!!!!!!!!
AMRUT S BHOGLE
- epitope size
Carl Saxinger
- serum replacements
AMRUT S BHOGLE
- help: neomycin-resistance gene
Jonathan W. Jarvik
- Cycle Sequencing
Jun Ishikawa
- eukaryotic expression vectors
Jim Gale
- Setup Questions re: PCR apparatus (plates/oil)
MARLENE SNYDER
- Gene Delivery - Asialoglycoproteins in Colon?
steven joseph chmura
- Quantitative Analysis on Western Blots
Uday Khosla
- serum replacements
Frederick Garbrecht
- sequencing at high temp
Matthew Thomas
- cDNAs & ORFs
Mark Bradley
- stoffel frag
Dan Diaz
- Copper-staining of proteins
Craig Marshall
- RNA from blood
Kanematsu Institute
- Plasmid map drawing programs
harley at uctvax.uct.ac.za
- Western Blotting with TransaBind
Ed Rybicki
- USS DNA and Mutagenesis
dnicker at vax.oxford.ac.uk
- Drying Nitrocellulose Blots??
mic255z at vaxc.cc.monash.edu.au
- Address of Oxford Glyco-Systems
hitt at sci.wfeb.edu
- Please, HELP. Recombinant plasmid 1 ug wanted. See inside...
Marina V Knyazeva
- U SS DNA
skspoidn at reading.ac.uk
- SS DNA and Mutagenesis (self-priming SOLUTIONS)
the End
- stoffel frag
PRAKASH at ACD.TUSK.EDU
- Amersham Rapid Hyb
Russ Wyborski
- Vent et.al polymerase for PCR
Zoltan Penzes
- cycle seq reply to B.L. Cohen (e-mail to U Glasgow failed)
jmv at canctr.mc.duke.edu
- Bovine corneal endothelial cells
Harry Mangalam
- Hybaid thermal cycler
Bill Melchior
- Vent et.al polymerase for PCR
BMA14432 at VAX1.UTULSA.EDU
- Bovine corneal endothelial cells
Dennis J. Templeton
- PCR amplifying vertebrate D-loop: multiple bands
Andrew McArthur
- Vent et.al polymerase for PCR
Ashok Aiyar
- Help with Enzymatic Calculations
Ramon 'Jammin' Cuevas
- U DNA mutagenesis
skspoidn at reading.ac.uk
- cooper-staining
ITABAJARA VAZ
- Problems with Promega Taq
Dr. Duncan Clark
- Storage of RNA
Peter Kristensen
- Windows DotPlot
Dan Davison
- Mutant Screening Pros&Cons
nickerson at molbiol.ox.ac.uk
- Hybaid thermal cycler
Jarmo Wahlfors
- USS DNA & mutagen. THANKS!!
dnicker at vax.oxford.ac.uk
- Spin columns - revisited
wetsel_r at msdisk.wustl.edu
- Spin columns - revisited
Ed Rybicki
- cloning and purity of DNA
Peter Thoren
- Windows DotPlot
Ramin Charles Nakisaramin
- Calling Spain!
Chris_Jones at vme.ccc.nottingham.ac.uk
- hydroxyurea
DAVID SCOTT
- PCR machine
Moira J. van Staaden
- Calling Spain!
Klaus.Matthaei at anu.edu.au
- E.coli x2913
bugg at mbcf.stjude.org
- PCR machine
Klaus.Matthaei at anu.edu.au
- Sequoia-Turner Model 450 fluorimeter (should I buy one?)
Richard Josephson
- Promega Taq, Summary
Patricia Foster
- Follow-up on Mouse Monoclonal Subtyping Assays
Shaun D. Black
- autorad cassettes
Michelle Waycott
- DNA oligo probe sequences for use with Insitu Hybridization.
morgue at angis.su.oz.au
- Cloning and purity of DNA
Jianchi Chen
- autorad cassettes
Jonathan B. Marder
- autorad cassettes
donald seto
- Promega Taq problems
Donna Jensen
- Storage of RNA
GIETZ at bldghsc.lan1.umanitoba.ca
- Mismatch specific nicking
Basavaraju Shankarappa
- fusion proteins in yeast and coli
The system I am looking at is made by Novagen, using a t7
Euan R. Taylor
- rna at -40 to -80 ?
suter at VAX.MPIZ-KOELN.mpg.d400.de
- gentamicin
Bob Ludwig
- Promega Taq, experiment
Patricia Foster
- Frequently Asked Question (FAQ) list for bionet.molbio.methds-reagnts
Paul N Hengen
- autorad cassettes
James B. Hutchins
- HELP!!Determing amino acid solution concentration
wujng xian
- Which amino acids can coli use as carbon sources?
cherry at watneys.med.utah.edu
- Promega Taq polymerase
Per Stalhandske
- Promegas Taq polymerase
Per Stalhandske
- Water Microbiology Workshop
Duncan Veal
- magnetic bead RNA preps
Gessler Manfred Dr.
- T7356- phage
Vic Ilag
- PFGE agarose plugs
John Nash
- third try- fusion proteins
Euan R. Taylor
- storage temp for riboprobes
sticknbd at miranda.cc.vanderbilt.edu
- PROMEGA TAG!!!!!!!!
Greg Hawkins
- Cetus's proof reading enzyme
Dr. Duncan Clark
- Ignore first posting, read this one!!!
Joy Richman
- Expressed protein extraction from cos cells?
Daniel Meier
- differential screening/display
Basavaraju Shankarappa
- PCR Buffers
Andrew McArthur
- credit for FAQ answers
Paul N Hengen
- fusion proteins - final try
Euan R. Taylor
- autorad cassettes (fwd)
Robert Preston
- Franklin Expedition
LMalburg
- PROMEGA TAG!!!!!!!!
Ed Rybicki
- Los Alamos DNA database (?)
Nico van Belzen
- PFGE
Mark Bradley
- Ethidium Bromide
Robert Horton
- Los Alamos DNA database (?)
Jim Owens
- Pharmacia BIAcore
Michael van Waes
- Ethidium Bromide
Sandy Silverman
- test
Thomas Vanaman
- Small, Cheap disulfide linked Protein
Mr. Christopher M. Gross
- Cyanobacteria...again
botbml at lure.latrobe.edu.au
- EtBr safety procedures
BLC
- search SOYBEAN GENOMIC LIBRARY
reichert at MIBM.RUF.UNI-FREIBURG.DE
- LPS Ab
Mr. D.J. Atherton
- Help! Separation of D- and L- arabinose
H.J.Treumann Biochemistry ext 4301
- Hybaid Thermal Cycler
PRAKASH at ACD.TUSK.EDU
- PCR software
Jim Gale
- PCR software
PWATSON at bldghsc.lan1.umanitoba.ca
- RNA gelshifts
GENE SIMON HUH
- Taq Sequencing
Daniel Mytelka
- The best CHEF electrophoresis conditions??
Nelson Salazar
- Promega Taq, Hint
Patricia Foster
- The best CHEF electrophoresis conditions??
Vivian Miao
- pasteur / cancer (was: etbr)
suter at VAX.MPIZ-KOELN.mpg.d400.de
- pasteur/cancer
suter at VAX.MPIZ-KOELN.mpg.d400.de
- yet more cycle sequencing
skspoidn at reading.ac.uk
- Native PAGE
Bill Richards
- NEN pen (Labelling autorads etc)
Andy Phillips
- low melt agarose and PFGE plugs
John Nash
- etbr waste
suter at VAX.MPIZ-KOELN.mpg.d400.de
- transgenic mice
hartla at dsbgpx.edvz.sbg.ac.at
- PFGE - agarose plugs (summary)
John Nash
- Gentamycin
THOMPAJ at GCRI.AFRC.AC.UK
- NEN pen (was: autorad cassettes)
Frederick Garbrecht
- Native PAGE
Ding Ming
- Chromosome Idiograms, human and mouse
David A. Adler
- EtBr safety procedures
Klaus.Matthaei at anu.edu.au
- EDS-76 sequence ?
chai_z at wehi.edu.au
- Inhibition of uncharacterized protease(s) in growth medium
VANNESTEJ%RUASV1 at ruakura.cri.nz
- sickness due to genetech ?
R.G. Walters
- disappearing PCR product
Christoph Schller
- sickness due to genetech ?
suter at VAX.MPIZ-KOELN.mpg.d400.de
- rat liver cytosol
Ed Stokes
- Phosphorescent Ink
shapirop at ohsu.edu
- Where do you buy Hygromycin?
APONCE at CINVESMX.BITNET
- Where do you buy Hygromycin?
APONCE at CINVESMX.BITNET
- Sequencing double-stranded RNA
Peter J. Russell
- Labelling by PCR
Jaishree Muppala Chittoor
- lacZ cassette
Peter J. Russell
- Labelling by PCR
David J. Meyer
- native PAGE
Peter
- Joyce-Leung Random PCR Mutagenesis
the End
- tRNAs and other stories
Paulo Magalhaes
- Thermal-stable polymerases
Jim Gale
- SMART System
Ding Ming
- Flourescein labeling- Help!
Sheila Gay Buzzee
- BIONET DNA Sequencing Survey
Andrew McArthur
- Flourescein -On
Basavaraju Shankarappa
- Flourescein labeling- Help!
Michele Fuortes
- RPA analysis
Brenda Leeuwenberg
- high background with dig in situ
sticknbd
- Geneclean
Carol Enderlin
- Average size of rescued plasmids with XbaI?
g78099a
- dissolving of SDS-PAGE bands
thomas at biomed.biolan.uni-koeln.de
- DIG_LABELLING
GJKING at NVRS.AFRC.AC.UK
- random priming in agarose
Wafa.El-Adhami at anu.edu.au
- Looking for E. coli dcm- strain
swilliam at mvax.cbm.uam.es
- EtBr Destruction Protocol
E85J000
- BIONET DNA Sequencing Survey
Rick Wilson
- DIG_LABELLING
James N Petitte
- EtBr Safety
metzler at sara.cc.utu.fi
- NEN pen
Sonia Pearsonwhite
- formaldehyde gels
John Jones
- formaldehyde gels
Xiaoying Lin
- AgNO3 DNA Staining Procedure Questions (David Atherton, are you there?)
TIM CHIPMAN
- duplicating film for Ag-stained seq gels
EM609MBCPHL at NCCCOT7.AGR.CA
- Speed-Vac rendundancy
the End
- RT-PCR: which enzyme(s) are best?
David J. Meyer
- BIONET DNA Sequencing Survey
pmiguel at bilbo.bio.purdue.edu
- dissolving of SDS-PAGE bands
James B. Hutchins
- Speed-Vac rendundancy
John Jones
- Speed-Vac rendundancy
John Nash
- RNase protection
DG at dna.bio.warwick.ac.uk
- Mitochondrial DNA isolation
Christoph Schller
- CsCl (formerly EtBr Safety)
c10_dcp2 at RED.WHOI.EDU
- EtBr Destruction Protocol
c10_dcp2 at RED.WHOI.EDU
- Geneclean
ww40
- EtBr ussage
brennanp at vax1.tcd.ie
- EtBr ussage
John Nash
- Malonic Acid
HARVEY at JII.AFRC.AC.UK
- ABI 373A question...
wetsel_r at msgcg.wustl.edu
- Promega's Silver Sequence Kit
P. Knygsman
- EtBr ussage
Stephen Gisselbrecht
- Inhibition of uncharacterized protease(s) in growth medium
Robert Preston
- glycoproteins
ITABAJARA VAZ
- EtBr ussage
Cheung C. Yue
- EtBr in gels
Nigel Walker
- mRNA purification
Jin Rong Xu
- Automatic and Semi automatic plate fillers
Ken Krauter
- overexpression with pGEX
batu erman
- Sequencing primer for pKK232-8
mic255z at vaxc.cc.monash.edu.au
- non-RI detection of nucleic acid
OKADA Yasushi
- mRNA purification
KARN at Butler.EDU
- random priming -->> increased probe size??
Basavaraju Shankarappa
- purification of T-even phages
Denni Schnapp
- random priming -->> increased probe size??
Basavaraju Shankarappa
- glycoproteins
James B. Hutchins
- BIONET DNA Sequencing Survey
pmiguel at bilbo.bio.purdue.edu
- Mitochondrial DNA isolation
Jim Cummins
- insoluble protein--need advise
gr8bn at cc.usu.edu
- BIONET DNA Sequencing Survey
Klaus.Matthaei at anu.edu.au
- plant genomic DNA iso and/or CTAB safety
SCHMID at Butler.EDU
- BIONET DNA Sequencing Survey
Mark Guiltinan
- Protein estimation in presence of detergents
cthomas at science.adelaide.edu.au
- EtBr usage
Ed Rybicki
- EtBr Safety / CsCl disposal / recycling
Ed Rybicki
- random priming
Wafa.El-Adhami at anu.edu.au
- sequenase on ABI machines
Rick Wilson
- Sequenase on the 373
Rick Wilson
- Sequenase on the 373
Rick Wilson
- recycled CsCl
c10_dcp2 at RED.WHOI.EDU
- Protein estimation in presence of detergents
Basavaraju Shankarappa
- DNA Sequence Pile-ups
Mark Guiltinan
- Suggestions needed for PCR
Ramon 'Jammin' Cuevas
- pUC18 question
Len Adleman
- Suggestions needed for PCR
Ed Rybicki
- DNA Sequence Pile-ups
Dr. M.D. Jones
- EtdBr cleanup - need advice
Larry Hale
- Salt Gradient Sequencing gels
Ethan Strauss
- Guessing DNA sizes after digestion
Antonio R. Franco
- CHEF Mapper system users
Mr. N.A. Salazar
- Gradient gels
NWALKER at JHUHYG.BITNET
- Suggestions needed for PCR
Paul N Hengen
- in vitro transcriptions
Mark Henteleff
- Salt Gradient Sequencing gels
Klaus.Matthaei at anu.edu.au
- Pre-cast SDS PAGE gels
M_COYNE at DELPHI.COM
- Non-radioactive mini or maxi cells?
M_COYNE at DELPHI.COM
- DNA Contamination in PCRs
farleyp at HAL.HAHNEMANN.EDU
- pGEX expression problems
DENNIS P. SMITH
- Northern - prehybr.
SEPPO KAIJALAINEN MMETT
- Opinions needeed about phosphoimagers
Ari-Pekka Kvist
- Address for PCR methods and applications!!!
Basavaraju Shankarappa
- Cleaning of V-Bottom Microplates used in PCR..?
TIM CHIPMAN
- E-mail addresses in publications
Shaun D. Black
- inclusion body mutant
Peter Alefounder
- HPLC Part
duhling at unix.cc.emory.edu
- Non-isotopic kits
Jamie Stanek
- pGEX expression problems
farleyp at HAL.HAHNEMANN.EDU
- mRNA extraction from tissue options ...
steven joseph chmura
- Problems with Promega silver sequencing kit
Xiu Yu
- UBI Primers
Phillip Lowrey
- PCR of translocated chromosomes
Jim Owens
- mRNA without main open reading frame?
chai_z at wehi.edu.au
- Yeast cDNA library
Stuart Clarkson
- DNA Contamination in PCRs
HoareMM at imb.lan.nrc.ca
- cosmid required
parkhillj at vax1.bham.ac.uk
- Supply of ball bearing "cell buster"?
mbxfd at unicorn.nott.ac.uk
- CTAB functionality as replacement for SDS..?
TIM CHIPMAN
- M13 mp2 mutants wanted
Zophonias Oddur Jonsson
- Deformation of Sequencing Gel
John Altman
- Q: on chloramphenicol resistance genes
Zophonias Oddur Jonsson
- RNA polymersase inhibitor
DAVID SCOTT
- yeast colony hybridization
Peter J. Russell
- DNA Contamination in PCRs
Terese M Barta-1
- Opinions needeed about phosphoimagers
Klaus.Matthaei at anu.edu.au
- EtBr Usage
Bob DeLisio
- affinity purifcn. Ab
Ed Rybicki
- Help with degenerate primers
Hugh Blackbourn Biochem
- mRNA from bacteria
metzler at sara.cc.utu.fi
- DNA from old bones
Andy Dizon
- DMS methylation
Adam Law
- DNA contamination in PCrRs
metzler at sara.cc.utu.fi
- EtBr Usage
metzler at sara.cc.utu.fi
- Copper Phenanthroline and Nuclear Protein
gvacano at eagle.wesleyan.edu
- Gram possitive lysis procedure
Raul Arredondo
- COS cell transfection...
wetsel_r at msgcg.wustl.edu
- Hybridisation oven questions
Nigel Walker
- control antisense for chickens
nishir at ohsu.edu
- Northerns/acrylamide
David Kling
- Phage host
Saeed Khan
- Inexpensive spin-columns
Lawrence P. Casson
- I want sequence of c-rel.
Kiichi Hirota
- F plasmid won't transfer
the End
- F plasmid won't transfer
Robert Preston
- GroEl chaperone commercial source?
Angelo Gunasekera
- Gel leaks (was Salt Gradient Gels)
M_COYNE at delphi.com
- Gel leaks (was Salt Gradient Gels)
Cornelius Krasel
- Gel filtration in urea or Gu-HCl?
Helmut Dotzlaw
- Viewing spinal motoneurons
Vic Ilag
- Hybridisation oven questions
Klaus.Matthaei at anu.edu.au
- Protein assays and detergents
cthomas at science.adelaide.edu.au
- nonradioactive seqn
brett at BORCIM.WUSTL.EDU
- seqn gel pouring
brett at BORCIM.WUSTL.EDU
- Gel leaks (was Salt Gradient Gels) (fwd)
Robert Preston
- F plasmid won't transfer
VANNESTEJ%RUASV1 at ruakura.cri.nz
- sequencing gel pouring
brknig01 at uctvax.uct.ac.za
- Protein estimation in presence of de
HoareMM at imb.lan.nrc.ca
- biosurfactants
ANNETTE CAHN VON SEELEN
- gel pouring w/o leaks
scarr at kean.ucs.mun.ca
- Hybridization oven question
LAZO,GERARD
- labelling in gel slice
bugg at mbcf.stjude.org
- CJ236 F' episome ?
the End
- Eukaryotic protein expression
awalley at molbiol.ox.ac.uk
- CSH118 strain and RSF2001 F'
the End
- cheap spin columns
Andy Dizon
- test, please ignore
David Kristofferson
- pGEX-fusions
Dr. S. Bhattacharya
- test of bionet.molbio.methds-reagnts - please ignore
David Kristofferson
- Isolation of asitic lymphocites
Raul Arredondo
- Gel filtration in urea or Gu-HCl?
Ding Ming
- pouring sequencing gels
Bruce Elder
- Ultracentrifugation of large #'s of small samples
HEPAD at TTACS1.TTU.EDU
- Inosine and E. coli
BOB RUTLEDGE
- PCR primers for murine T cell
Peter Kuhlman
- Transfer of genes to E.coli chromosome
Dr. Duncan Clark
- Pouring sequencing gels w/o leaking
Tae Shin
- cross-hybridization in T7 RNA assay
rla at canctr.mc.duke.edu
- gene walking techniques
APPLING at UTBC01.CM.UTEXAS.EDU
- Luciferase in transgenic experiments
Ari-Pekka Kvist
- Marker for fibroblasts _in vitro_?
Garrett H. Riggs
- Seeking peptide sources
Mark Mattson
- Protein A - References
ottcr at esvx17.es.dupont.com
- Visualization of DNA in agarose gels (EtBr alternatives..?)
TIM CHIPMAN
- HELP
Dr. Duncan Clark
- Silver Stain
Mr. Christopher M. Gross
- Help: Lambda Sequencing
sarkari at mpimg-berlin-dahlem.mpg.de
- Silver Stain::Destaining
Basavaraju Shankarappa
- Help on electroporation of Swiss 3T3 cells
All FMI-users
- k-ras cDNA
bugg at mbcf.stjude.org
- Isolation of oviduct mRNA
AXC19 at psuvm.psu.edu
- More Hybridisation ovens!
Nigel Walker
- cleaning up PCR reactions
Andy Dizon
- GM-CSF doses?
PECHAN at HELIX.MGH.HARVARD.EDU
- Et-Br disposal
CARLOS ALVAREZ
- PCR troubleshooting
Terese M Barta-1
- Need pKK223-3 vector
Steven Joseph Szarka
Last message date:
Wed Jun 30 23:38:29 EST 1993
Archived on: Sun Mar 6 19:51:30 EST 2005
This archive was generated by
Pipermail 0.09 (Mailman edition).