Problems with new Sequenase?

Dan Diaz bl275 at cleveland.Freenet.Edu
Mon Mar 1 19:18:19 EST 1993

In a previous article, pmiguel at () says:

>  Has anyone had troubles with Sequenase since USB introduced their
>new Glycerol Enzyme Dilution Buffer?  I mean the tubes of Sequenase 
>which are packaged with reaction (annealing) buffer which has a
>blue label on the top.
>  A couple of people in our lab have had very faint bands on their
>autorads (maybe 5x fainter than normal).  We have new 35S-dATP and one 
>of them was working with templates that previously gave him no
>  We have not been using the new Glycerol Enzyme Dilution Buffer which 
>requires the use of Taurine, rather than Borate, buffered gels.

Silly point, I know, but I assume that you have already called USB and
asked them about your problem.
Dizzy Dan         ddiaz at cwru.bitnet      bl275 at
Department of Molecular Genetics and Curried Sauces
Albert Einstein College of Medicine, Da Bronx, New York

More information about the Methods mailing list