Problems with new Sequenase?

Dan Diaz bl275 at cleveland.Freenet.Edu
Mon Mar 1 19:18:19 EST 1993


In a previous article, pmiguel at bragg.bio.purdue.edu () says:

>  Has anyone had troubles with Sequenase since USB introduced their
>new Glycerol Enzyme Dilution Buffer?  I mean the tubes of Sequenase 
>which are packaged with reaction (annealing) buffer which has a
>blue label on the top.
>  A couple of people in our lab have had very faint bands on their
>autorads (maybe 5x fainter than normal).  We have new 35S-dATP and one 
>of them was working with templates that previously gave him no
>trouble.
>  
>  We have not been using the new Glycerol Enzyme Dilution Buffer which 
>requires the use of Taurine, rather than Borate, buffered gels.

Silly point, I know, but I assume that you have already called USB and
asked them about your problem.
-- 
Dizzy Dan         ddiaz at cwru.bitnet      bl275 at cleveland.freenet.edu
Department of Molecular Genetics and Curried Sauces
Albert Einstein College of Medicine, Da Bronx, New York



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