T-vector prep via fillin

BOB RUTLEDGE BRUTLEDGE at ZEKE.PNFI.FORESTRY.CA
Mon Mar 1 10:51:53 EST 1993


In view of the difficulties of preparing T-vectors using Taq, we are considering a partial fill in approach using Klenow (+dC, dG, dT) and M13mp18/19 cut with SalI.

Has anyone attempt to use this approach for preparing T-vector for cloning PCR amplified DNA?

Bob Rutledge
Petawawa National Forestry Institute
brutledge at zeke.pnfi.forestry.ca



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