In view of the difficulties of preparing T-vectors using Taq, we are considering a partial fill in approach using Klenow (+dC, dG, dT) and M13mp18/19 cut with SalI. Has anyone attempt to use this approach for preparing T-vector for cloning PCR amplified DNA? Bob Rutledge Petawawa National Forestry Institute brutledge at zeke.pnfi.forestry.ca