Slab IEF

MBB MBB
Tue Mar 2 04:33:49 EST 1993


I am doing isoelectric focusing on denaturing (6M urea) slab gels.
I am looking at two proteins of pI 7 and 6, or so.  The upper
band (pI 7) is always blurry, not well focused.  A complex mixture of
proteins shows that all the bands are not well focused in the top 
1/3 or so of the gel.  Could this be due to heat?  What else?

Steve Potter
UCI Psychobiology
Irvine, CA 92717
spotter at darwin.bio.uci.edu



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