Dephosphorylation

Angelo Gunasekera angelo at phoenix.Princeton.EDU
Tue Mar 2 01:34:05 EST 1993


we have successfully used Shrimp Alkaline Dephosphatase to
dephosphorylate 5' phosphate.  The best thing about it is that it can be
irreversibly denatured by heating the reaction mix. to 70c for few
minutes.  You don't even have to do a phenol extraction to remove the
SAP as it will all be inactive.  We buy SAP from USB.
Good Luck
Angelo



More information about the Methods mailing list