G-Nome kit (BIO 101)

BENNY SHOMER 9238 pc386 at tau.ac.il
Thu Mar 4 08:38:05 EST 1993


Hi Bionetters.

Darin O'Brien (obrien at molbio.cbs.umn.edu) wrote:
: Hi!

: 	I was wondering if anyone out there has tried the G NOME kit by
: BIO 101 for isolation of genomic DNA from mammalian tissue culture cells.
: I have tried but have had little success.  Starting with 1-2 X 10^7 cells,
: I end up with a precipitate after salting out that won't pellet very well.  The
: DNA is supposed to be in the supernatant but I can't get much of anything
: to precipitate.  Presumably my DNA is still in the lose gunky pellet with the
: protein.  Any suggestions or comments would be greatly appreciated.

: Thanks much,

: Darin (obrien at molbio.cbs.umn.edu)

I have been using G NOME kit for isolations either from cultured fibroblasts, 
or from murine tail pieces. I have allways obtained high quantities of high
quality G-DNA. I have performed the procedure on the same amounts of cells,
by spooling on a glass rod, but I also successfully downscaled the reaction
by a 1:10 ratio, collecting the DNA by spinning.
Are you certain there's no degradation of your DNA? Do you regularely obtain
G-DNA from the same cultures by "normal" Phenol extractions ? 

As allways: I'm not associated with BIO-101 etc.

Sincerely, Benny.
 **************************************************************
* Benny Shomer                                                 *
* Tel-Aviv University                                          *
* Sackler School of Medicine, Dept.of Embryology and Teratology*
*--------------------------------------------------------------*
* Snail:  Ramat-Aviv , Tel-Aviv  69978 ,  Israel.              *
* E-mail :  pc386 at ccsg.tau.ac.il                               *
* Tel :  972-3-640-9238     FAX :   972-3-642-2046             *
*                                                              *
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