Staining DNA on nylon?: MW markers
BRUTLEDGE at AM.PNFI.FORESTRY.CA
Thu Mar 4 17:18:15 EST 1993
In <1993Feb26.124033.770 at otago.ac.nz> bioc07 at otago.ac.nz
> By an act of omission, an acrylamide gel containing DNA was
> electroblotted before the DNA could be stained with EthBr. Is there
> easy way to stain the membrane after Southern blotting to get the
> position of the markers and such.
I suggest that EthBr staining (for locating MW markers) can be
avoided totally by simply running end-labelled MW markers (Maniatis)
which then produce bands directly on the autorad. We rountinely
do this for all of our DNA blots.
Petawawa National Forestry Institute
brutledge at pnfi.forestry.ca
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