Staining DNA on nylon?: MW markers

BOB RUTLEDGE BRUTLEDGE at AM.PNFI.FORESTRY.CA
Thu Mar 4 17:18:15 EST 1993


In <1993Feb26.124033.770 at otago.ac.nz> bioc07 at otago.ac.nz 
writes:
> By an act of omission, an acrylamide gel containing DNA was
> electroblotted before the DNA could be stained with EthBr. Is there 
an
> easy way to stain the membrane after Southern blotting to get the
> position of the markers and such. 

I suggest that EthBr staining (for locating MW markers) can be 
avoided totally by simply running end-labelled MW markers (Maniatis) 
 which then produce bands directly on the autorad.  We rountinely 
do this for all of our DNA blots. 

Bob Rutledge
Petawawa National Forestry Institute
brutledge at pnfi.forestry.ca




More information about the Methods mailing list