salicylate & fluorography
BRUTLEDGE at AM.PNFI.FORESTRY.CA
Thu Mar 4 16:53:09 EST 1993
>I'm looking for both a protocol and a reference for
>the use of sodium salicylate as a fluor for H3, S35,
>and C14 labelled protein acrylamide gels.
>A partial protocol I found suggests soaking in water
>and 1M sodium salicylate.
>Does anyone have more details or a reference to this?
I have sucessfully used salicylate for C14 fluorography. Following 30
mins. in distilled water, the gel is treated for 30 mins in 1.0 M sodium
salicylate and dried. The reference is:
Chamberlian, J.P. 1979. Flourography detection of of radioactivity in
polyacrylamide gels with the water-soluble fluor, sodium salicylate.
Anal. Biochem. 98, 132.
Petawawa National Forestry Institute
brutledge at pnfi.forestry.ca
More information about the Methods