salicylate & fluorography

[Mark Strom]

russell at DOGWOOD.BOTANY.UGA.EDU (Russell Malmberg) writes:

>I'm looking for both a protocol and a reference for
>the use of sodium salicylate as a fluor for H3, S35,
>and C14 labelled protein acrylamide gels.

I've used 0.5 M salicylate-1.5% glycerol after the water wash for
H3-fluorography with good success.  The gels don't crack as readily during
drying and the signal obtained was strong. (ref. Sambrook, et al. 1989.
Molecular Cloning: a laboratory manual). 

-- 
Mark S. Strom, Ph.D.                  	     office: (206) 553-4182
UR Division, NW Fisheries Sci. Ctr.             lab: (206) 553-7707
Natl. Marine Fisheries Ser., NOAA	        fax: (206) 553-4304
2725 Montlake Blvd. E.,  Seattle, WA  98112   email: strom at u.washington.edu