salicylate & fluorography
strom at stein.u.washington.edu
Fri Mar 5 13:22:50 EST 1993
russell at DOGWOOD.BOTANY.UGA.EDU (Russell Malmberg) writes:
>I'm looking for both a protocol and a reference for
>the use of sodium salicylate as a fluor for H3, S35,
>and C14 labelled protein acrylamide gels.
I've used 0.5 M salicylate-1.5% glycerol after the water wash for
H3-fluorography with good success. The gels don't crack as readily during
drying and the signal obtained was strong. (ref. Sambrook, et al. 1989.
Molecular Cloning: a laboratory manual).
Mark S. Strom, Ph.D. office: (206) 553-4182
UR Division, NW Fisheries Sci. Ctr. lab: (206) 553-7707
Natl. Marine Fisheries Ser., NOAA fax: (206) 553-4304
2725 Montlake Blvd. E., Seattle, WA 98112 email: strom at u.washington.edu
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