Beta-Agarase and PCR

r.nakisa at r.nakisa at
Fri Mar 5 16:22:22 EST 1993

Dear Netters,

I'm using beta-agarase to isolate a ligation product of two linear
fragments.  The fragments have sizes of 1000 and 400 b.p., and I cut
the 1400 ligation product out of a 1% LMP gel and agarase digest.
Then I spin down the undigested agarose, pipette the supernatent into
a new eppendorf and precipitate with isopropanol according to the

But here's the crunch.  When I try to amplify the 1400 b.p. fragment,
it doesn't amplify at all!  Does anyone know whether Taq polymerase is
affected by the products of an agarase digestion?

< Ramin Charles Nakisa, "She walks in beauty Tel:    071 589-5111 x 6729   >
> Biophysics Section,   As the night of      FAX:    071 589-0191          <
< The Blackett Lab,     starry skies and     EMAIL:                        >
> Imperial College,     cloudless climes..."         ramin at        <
< London SW7 2BZ.                                    mbrcn at >

More information about the Methods mailing list