large scale antibody production

Frederick Garbrecht fgarbrec at POST.ITS.MCW.EDU
Mon Mar 8 12:55:08 EST 1993

On 8 Mar 1993 rcwieg at wrote:

> Date: 8 Mar 93 16:20:52 GMT
> From:rcwieg at
> To: methods-and-reagents at
> Subject: Re: large scale antibody production
> In article <Pine.3.05.9303051806.A10732-a100000 at>
> fgarbrec at POST.ITS.MCW.EDU (Frederick Garbrecht) writes:
> >I am interested in scaling up hybridoma production of monoclonal
> >antibodies to produce quantities of antibody on the order of GRAMS.  Have
> >experimented with Amicons mini-flo-path bioreactors with good success,
> but
> >would like to use a more full featured, self-contained unit.  Has anyone
> >out there experience with the use of any of the larger scale hollow fiber
> >bioreactors, or any other technology for that matter, that would
> >facilitate very large scale Ab production?  Also, if anyone is aware of a
> >biotechnology group on the net, where this type of post might be more
> >appropriate, please let me know.
> >Thanks,
> >Fred Garbrecht
> Is there some reason you don't want to make ascites? I don't know of a
> better or cheaper bioreactor than a mouse if all you need is a gram or two
> of antibody.
> Roger Wiegand
> rcwieg at
> Monsanto Corporate Research
> Chesterfield, MO

I need many grams, and since this would be for potential in vivo use it
needs to be nice and pure; ascites has many disadvantages in this regard.

Fred Garbrecht
Medical College of Wisconsin
fgarbrec at

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