UGG00146 at VM.UOGUELPH.CA
Thu Mar 11 11:53:59 EST 1993
We want to confirm the size of an RNA fragment that we predict is
310-315 bases. I ran this RNA beside a sequencing ladder and it
migrates next to a 340 base DNA fragment. Of course, I expect the RNA to
run slower than DNA, but I don't know how much slower. The gel includes
some 5.8S rRNA, which is known to be 158 bases, and it runs like a 166 base
DNA fragment. From this I calculate that RNA runs about 5% slower than DNA,
but I am not sure if this can be assumed for different sized RNAs. For the
RNA that I am interested in, the size estimate is about 3% off my prediction.
How should I handle this problem????
Is there a better sizing method????
U o Guelph
More information about the Methods