5'->3' exonucleases

Carol Enderlin enderc at essex.hsc.colorado.edu
Thu Mar 11 21:35:20 EST 1993


In article <1993Mar8.182657.1 at msscc.med.utah.edu> ctwittwer at msscc.med.utah.edu writes:
>Exonuclease III from E. coli won't touch a 3' overhang.  I'd like to use an
>exo which won't touch a 5' overhang. 

This may not be quite what you are looking for, but
ExoIII will not "touch" a 5' overhang that has been
filled in (Klenow) with thio-dNTPs.



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