Designing GC Rich Oligos

u6064043 at u6064043 at
Fri Mar 12 23:17:27 EST 1993

Hi netters,

I'm working on a plant protein which is very rich in Proline, Serine, Alanine
etc. The aim of my work is to clone the gene from the protein sequence. So 
here is the problem: Pro, Ala codons are high in GC content, and to make
it worse, they have 4-6 degeneracies. Imaging a primer having >80% GC and 
>500 degeneracies! So far it proves to be difficult to do PCR and even when
there is a single PCR band, it turn out to be something completely meaningless.
This protein is not homologous to other cloned genes, such as extensin, apart
from the Proline stretch. Any suggestions?


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