Your fav Phosphatase?

GIETZ at bldghsc.lan1.umanitoba.ca GIETZ at bldghsc.lan1.umanitoba.ca
Sun Mar 14 19:26:00 EST 1993


Hi Diz!
    One question about the shrimp enzyme.How effective is the
dephosphorylation reaction?  What are your % inserts, lets' say 
 into a pUC vector?  (Blues vs Whites).  Can you expect 90 to 95%
inserts under good ligation conditions?  I'm wondering if I should 
switch?
________________________________________
Dan Gietz
Department of Human Genetics
University of Manitoba
770 Bannatyne Ave, Rm 250
Winnipeg, Man, Canada
R3E 0W3
GIETZ at BLDGHSC.LAN1.UMANITOBA.CA
_________________________________________



More information about the Methods mailing list