Quantitative RT-PCR

gvacano at eagle.wesleyan.edu gvacano at eagle.wesleyan.edu
Wed Mar 17 14:12:48 EST 1993


Hello--

	I am interested in quantitating transcription of alleles introduced
into Drosophila by P-element mediated transformation, relative to the native
gene expression, using an RT-PCR method. Any references, protocols, kit
information, and/or first-hand experiences will be deeply appreciated.

Thank you, Guido Vacano
	   Dept. of Mol. Biol. and Biochem.
	   Wesleyan University
	   Middletown, CT 06459-0175
	



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