bandshift and no footprint help

IBELGAUFTS at vms.biochem.mpg.de IBELGAUFTS at vms.biochem.mpg.de
Thu Mar 18 02:24:51 EST 1993


dear netters
We have a DNA binding protein. It causes a band shift with an appropriate
oligo and a DNA fragment of approx. 200 bp containing its recognition
sequence.
although a distinct shift is seen with methzlated DNA in mobilitz shift
assays we do not get a footpring bz methylation interference. We also cannot
get a DNAse footprint from DNAse-treated DNA cut out from a retention gel and
subsequently used for footprinting.
Any suggestions for an explanation??

thanks in advance

Horst ibelgaufts



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