precipitation of oligos

chai_z at wehi.edu.au chai_z at wehi.edu.au
Sun Mar 21 22:01:39 EST 1993


In article <UfeSrrS00Uh_I2YVpl at andrew.cmu.edu>, "Anand K. Bachhawat" <ab4u+ at andrew.cmu.edu> writes:
> Dear netters,
> 1) What is the best method to get 17-mer and 20-mer oligos in aqueous
> solution (that have been used for sequencing) back into a powder form?
> 2) What kind of recovery does one get when one precipitates 17-mer and
> 20-mer oligos using (a) ethanol and (b) isopropanol ? Are there any
> carriers that would help in getting a better recovery? 
> Thanks in advance
> Anand Bachhawat

There is a paper you may refer to: Paithankar & Prasad (1991) Precipitation of
DNA by polyethylene glycol and ethanol. Nucleic Acid Research 19(6):1346.

	They precipitate the HindIII-digested pUC13 DNA using 2 vol. ethanol
and 10mM MgCl2 at room temperature for 10min and the smallest fragment of the
digestion (26mer) was precipitated. 

	Please note that the fragment in the paper were dsDNA while your oligos
are single stranded.

Good Luck.

Zhonglin Chai
Melbourne



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