MAAPing suggestions?

Dr. J. Lorenzen akl01 at rs1.rrz.Uni-Koeln.DE
Thu Mar 25 11:37:21 EST 1993


I am just starting out on a research project to investigate cytokine
expression patterns from few (or single?) cells. Data on amplification
of DNA from single cells have been quite promising so far. Does anyone
have experience in RT-PCR (or the MAAPing-technique for that matter)?
How do you prepare RNA from small samples?
Should I use Superscript or rTth?
All suggestions welcome!
J. Lorenzen
Department of Pathology
University of Cologne
E-Mail: akl01 at rs1.rrz.uni-koeln.de



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