Has anyone isolated mRNA from rough ER?

[Michiaki Masuda]

   Dear b.m.m-r netters:

   I'm looking for a good protocol for isolating mRNA from rough ER of
cultured cells for cDNA cloning. If you have a protocol that is working 
successfully or any idea which articles to refer to, I'd appreciate your
information.

   Thank you.

Michiaki Masuda
Laboratory of Molecular Oncology
National Cancer Institute
Frederick, MD 21702-1201
U.S.A.
E-mail: Masuda at ncifcrf.gov
Phone: (301) 846-5740
F A X: (301) 846-6164