Methods March 1993 Archives by date

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Starting: Mon Mar 1 00:08:00 EST 1993
Ending: Wed Mar 31 19:07:02 EST 1993
Messages: 433

Question: Genomic Libraries J Preiss--Seq Anal
Help with immunoprecipitation V. Warwar
improve the signal in Northern ? Andre Hamel
Site directed mutagenesis oligo design Andre Hamel
blunt end ligations Rick Wilson
Help with immunoprecipitation Basavaraju Shankarappa
blunt end ligations (and libraries) Francis Ouellette
T-vector prep via fillin BOB RUTLEDGE
Site directed mutagenesis oligo desi Ken Baker
Membranes, proteins and RNA Gordan Lauc
Problems with new Sequenase? PHILLIP_SANMIGUEL
(none) Richard Davis
Problems with new Sequenase? George W Chacko
Membranes, proteins and RNA Jon Binkley
Source for CEE JEMERTON at BIOTECHNET.COM
problems with XmaI digestion Basavaraju Shankarappa
nuclear run on BXCZERNI at UAMS.bitnet
Problems with new Sequenase? Dan Diaz
Using PRIMER-- a software selecting primers Jason Lei
Molec. Bio help (needed) pickett at kenyon.edu
bacitracin LOWDEN,Darren
Dephosphorylation Angelo Gunasekera
Slab IEF MBB
Problems with new Sequenase? vernon
why M13? Rick Wilson
Molec. Bio help (needed) Brain Foley
Dephosphorylation both lab
Dear all, Brain Foley
Nensorb20 query John Alsobrook
Rotem Industries J.F. Norman
Blunt end ligation summary Daniel Meier
Staining DNA on nylon? John Alsobrook
NEW Sequencing Gel Problem Greg Hawkins
Dear all, Mr. K.R. Raj
NEW Sequencing Gel Problem pmiguel at bragg.bio.purdue.edu
Molec. Bio help (needed) John Boswell
Problems with new Sequanase? siddiqui at uwovax.uwo.ca
problems with XmaI digestion Andre Hamel
Dephosphorylation Andre Hamel
Need source for KLH (pharmaceutical grade)
G-Nome kit (BIO 101) Darin O'Brien
Dear all, Andre Hamel
NEW Sequencing Gel Problem Andre Hamel
Molec. Bio help (needed) James B. Hutchins
Hirt Method for small DNA IVAN at MNI.lan.mcgill.ca
Molec. Bio help (needed) Joshua Kelly
Molec. Bio help (needed) KELLY THOME
PCR 4.5kb plasmid Angelo Gunasekera
Molec. Bio help (needed) Mart Speek
Rotem Industries Nir Navot
magnetic M13 preps Rick Wilson
2 Rounds of PCR Henrik Wengholt
PCR/BglII digestion problems FRANK,BART
Help with antibiotic detection KASUGAMYCIN W. Scott Laughinghouse
RT-PCR from a single cell? Randy Hardy
RT-PCR Using Oligo-dT Primer bhjelle at carina.unm.edu
2 Rounds of PCR Basavaraju Shankarappa
2 Rounds of PCR the End
PCR/BglII digestion problems Andre Hamel
2 Rounds of PCR Andre Hamel
NEW Sequencing Gel Problem Steven Brenner
Staining DNA on nylon? Steven Brenner
bacitracin LOWDEN,Darren
Staining DNA on nylon? Paul N Hengen
How do I get off this mailing list? Skott
RT-PCR Using Oligo-dT Primer Ed Rybicki
Problems with new Sequenase? brknig01 at uctvax.uct.ac.za
PCR 4.5kb plasmid norm eberhardt
G-Nome kit (BIO 101) BENNY SHOMER 9238
Phosphoimagers/Betascopes Jeffrey Alan Silverman
PC framegrabber for documenting ethidium gels. Chris Porter
Problems with new Sequenase? Ayeda Ayed
Classification of teratogens help needed Mark Juric [MSAI]
RT-PCR from a single cell? Andre Hamel
NEW Sequencing Gel Problem Andre Hamel
"Raytide"(?) synthetic peptide -- source? Mike Fessler
Classification of teratogens help needed Dat Trieu Le
Dephosphorylation GIETZ at bldghsc.lan1.umanitoba.ca
tritium scanners Ruud at sicsun.epfl.ch
In situ hybrid. to tissue with oligos ? Christophe Roos
Protocol request... Tao Tao
salicylate & fluorography Russell Malmberg
salicylate & fluorography BOB RUTLEDGE
Staining DNA on nylon?: MW markers BOB RUTLEDGE
Phosphoimagers/Betascopes wmelchior at ntet.nctr.fda.gov
5' phosphorylation reagent for oligos wardp at skyfox
Dephosphorylation Martin Kennedy
Origin of SEVAG? TGV at psuvm.psu.edu
Classification of teratogens help needed OPIRG
PCR/BglII digestion problems chai_z at wehi.edu.au
Origin of SEVAG? Pierre Jelenc
Novo Address RPSCOTT
salicylate & fluorography ryan at mbcf.stjude.org
Classification of teratogens help needed Mark Juric [MSAI]
Origin of SEVAG? wmelchior at ntet.nctr.fda.gov
salicylate & fluorography ryan at mbcf.stjude.org
wheaton bottles! nhi336 at aberdeen.ac.uk
Subscription request Stacey A Kraemer
Mutagenesis via PCR (who's using it ?) the End
Dephosphorylation Andre Hamel
Novo Address Ole N Jensen
NEW Sequencing Gel Problem Paul N Hengen
salicylate & fluorography Mark Strom
Sequencher program for ABI373 data scarr at kean.ucs.mun.ca
Repressed pTac leaks ! (Better ?) the End
salicylate & fluorography Vivian Miao
Beta-Agarase and PCR r.nakisa at ic.ac.uk
Miller Units definition GIETZ at bldghsc.lan1.umanitoba.ca
Soybean genomic library for free? Mark Schweder
large scale antibody production Frederick Garbrecht
Miller Units definition M.J. Horsfall
Sequencher program for ABI373 data Francis Ouellette
Miller Units definition Donald Chen - Microbiology
wheaton bottles! Andre Hamel
Beta-Agarase and PCR Andre Hamel
oligo purification jurgenson at iscsvax.uni.edu
wheaton bottles! TorrieJP at imb.lan.nrc.ca
CEPH/Genethon PCR conditions greg at MENDEL.LLNL.GOV
maps from database sequences GENE SIMON HUH
Dephosphorylation Martin Kennedy
Beta-Agarase and PCR Vivian Miao
Repressed pTac leaks ! (Better ?) G. SCOTT GORDON
NEW Sequencing Gel Problem Andre Hamel
Dephosphorylation Andre Hamel
Molecular Genetics teaching materials Andrew Hobbs
mtDNA methods Karyn Davis
wheaton bottles! Bill Melchior
Sequencher program for ABI373 data Rick Wilson
35-S Amersham George W Chacko
Protein A-Magnetic Beads?? Douglas Barker
PCR and Agarase r.nakisa at ic.ac.uk
Phosphoimagers/Betascopes Ola Myklebost
large scale antibody production rcwieg at ccmail.monsanto.com
large scale antibody production Frederick Garbrecht
Miller Units def: Thanks GIETZ at bldghsc.lan1.umanitoba.ca
pGEM 7 Zf+ sequence needed both lab
Survey: does maltose help or hurt lambda plaque size? David Steffen
Thermocycler Review Anyone? George W Chacko
Repressed pTac leaks ! (Better ?) Paul N Hengen
Need GHRH peptide seqeunce books at mcvax2.d48.lilly.com
5'->3' exonucleases ctwittwer at msscc.med.utah.edu
2 Rounds of PCR chai_z at wehi.edu.au
salicylate & fluorographyREAD/NEW/FOLLOWUP marder at agri.huji.ac.il
Repressed pTac leaks ! (Better ?) the End
Arabinose promoter vector ?? (Re:pTac leaks) the End
CJ236 plasmid pCJ105?? Warren Wakarchuk
PCR and Agarase davis at neb.com
Survey: does maltose help or hurt lambda plaque size? Andre Hamel
5'->3' exonucleases Andre Hamel
CJ236 plasmid pCJ105?? Andre Hamel
BglII/PCR digestion problems FRANK,BART
Can we avoid photographing gels? FARMERJ at BYU.EDU
Hoeffer Minflourometer S*CKS the End
Detecting fluorescein-labeled PCR fragments? FARMERJ at BYU.EDU
Why are gels shown running "down"? James R. Mensch Jr.
Hoeffer Minflourometer S*CKS Steve L. Moseley
Hoeffer Minflourometer Thomas Mitchell-Olds
<None> dcoleman at molbiol.ox.ac.uk
protein detection Anna Sessler
Can we avoid photographing gels? RICK REST, HAHNEMANN UNIV, PHILADELPHIA
Minifluorometer Terese M Barta-1
Hoeffer Minflourometer S*CKS the End
Hoeffer Minflourometer Toby Bradshaw
Hoeffer Minflourometer S*CKS Steve L. Moseley
Sea Urchin Oocyte DNA prep (query) Christopher Kashina Patil
metaphase chromosomes dcoleman at molbiol.ox.ac.uk
2,2' dipyridyl disulphide - solubility? Dave Pearton Biochemistry
Sea Urchin Oocyte DNA prep (query) Francis Ouellette
Need a 28S rRNA probe Edward Morgan
cancellation TAYLOR at UCS.INDIANA.EDU
Request for help with transfection of hepatocytes Edward E. Cable
subscribe Timothy C. Quick - postdoc - ext 4042
Phosphoimagers/Betascopes Bruce Byrne
Help with Adapters? Steven Joseph Szarka
How to isolate proteins from plant Feng Bai
Hoeffer Minflourometer S*CKS Daniel Meier
Survey: does maltose help or hurt lambda plaque size? Dr. S.A.J.R. Aparicio
Your Favorite Phosphatase? GIETZ at bldghsc.lan1.umanitoba.ca
Removal of EPS from gm -'ve cells Fintan VanOmmen Kloeke
Detecting fluorescein-labeled DNA in gels? farmerj at yvax.byu.edu
Sea urchin EGG DNA (query) Christopher Kashina Patil
pGEM 7 Zf+ sequence needed rpgrant at molbiol.ox.ac.uk
35-S Amersham rpgrant at molbiol.ox.ac.uk
Please teach me subtraction of mRNA. Kiichi Hirota
Your Favorite Phosphatase? Ashok Aiyar
Hoeffer Minflourometer NSTEWART at VTVM1.CC.VT.EDU
RNA sizing liam Good
Help with Adapters? Paul N Hengen
Miniflourometer (SUMMARY) the End
Minifluorometer FORNACE at FCRFV2.NCIFCRF.GOV
Detecting fluorescein-labeled PCR fragments? Andre Hamel
Frequently Asked Question (FAQ) list Paul N Hengen
5'->3' exonucleases Eric C. Kofoid
Sulphate uptake in E.coli David.Mann at anu.edu.au
Hoeffer Minflourometer S*CKS Martin Kennedy
Hoeffer Minflourometer S*CKS Martin Kennedy
<None> Martin Kennedy
Hoeffer Minflourometer S*CKS Terese M Barta-1
Brain cDNA library michael_m at vcp1.vcp.monash.edu.au
5'->3' exonucleases Carol Enderlin
Help with adapters sjszarka at ACS.UCALGARY.CA
Point Mutation Frequency Michael Lush
Phosphoimagers/Betascopes cherry at OPAL.MGH.HARVARD.EDU
Non-automated sequencing apparatus Laura Via
Removal of EPS from gm -'ve cells Paul N Hengen
northern sensitivity Blair Jennings
Biology and chaos Michael van Waes
Sequencher program for ABI373 data Jason E Stewart
oligo purification Jason E Stewart
Terminase Vendor pmiguel at bragg.bio.purdue.edu
Terminase Vendor PHILLIP_SANMIGUEL
Odd shaped protein band! cup at bones.biochem.ualberta.ca
Transforming Fungi Mark S. Rose
Your Favorite Phosphatase? Dan Diaz
Designing GC Rich Oligos u6064043 at ucsvc.ucs.unimelb.edu.au
Sequencher program for ABI373 data Bruce Roe
M13-genomic library-XL1-Blue-electroporation Phillip Lowrey
Sequencher program for ABI373 data Jason E Stewart
ELISA reader purchase advice request Hsing-Tsu Lai
Northern sensitivity RPSCOTT
M13-genomic Library-XL1-Blue-electr.. GIETZ at bldghsc.lan1.umanitoba.ca
Your fav Phosphatase? GIETZ at bldghsc.lan1.umanitoba.ca
Terminase Vendor Martin Kennedy
Terminase Vendor Andre Hamel
oligo purification Vivian Miao
M13 banking BOB RUTLEDGE
Membrane and Nucleic acid interaction?? I-shiou Ng
Biotechniques archive? Mark S. Rose
oligo purification Klaus.Matthaei at anu.edu.au
Cleaning electroporation cuvettes TLANE at watson.princeton.edu
Cleaning electroporation cuvettes Angelo Gunasekera
help HOMOLOGY program within INSIGHT Angelo Gunasekera
help HOMOLOGY program within INSIGHT Angelo Gunasekera
mtDNA methods Victor V. Levenson
Migration of Chromosomal vs. plasmid DNA Peter M. Muriana
5'-RACE Martyn White
Does the addition of maltose help phage lambda growth? David Steffen
RNA sizing Euan R. Taylor
Avian sex-determintion Bruce Elder
oligo purification Martin Kennedy
repost of Bgl ll/pcr problem
Migration of Chromosomal vs. plasmid DNA Martin Kennedy
Migration of Chromosomal vs. Plasmid DNA RPSCOTT
Q. About Gyrase Jun Ishikawa
Software wanted for densitometry Gast
wanted e-mail address Chuan-Shan Shiu, Arthur
Cleaning electroporation cuvettes Jim Oberwetter
Quantitative RT-PCR gvacano at eagle.wesleyan.edu
down 5000 bma14432 at vax1.utulsa.edu
ammonium persulfate in sequencing gels Bill Melchior
Cleaning Electroporation Cuvettes (summary) David Nunn
Migration of Chromosomal vs. plasmid DNA Terese M Barta-1
IgM isoelectric point Corinne L Reimer
bandshift and no footprint help IBELGAUFTS at vms.biochem.mpg.de
Biotech Job in Singapore Wong Wui Ming
Biotech Job in Singapore Lakshman Ramamurthy
Request tips for vacuum blotting of RNA Franck Brunel
ammonium persulfate in sequencing gels Basavaraju Shankarappa
M13 banking Paul N Hengen
GC content questions JAB5 at VAXB.YORK.AC.UK
quant. RT PCR Andre Hamel
test-please ignore JAB5 at VAXB.YORK.AC.UK
Getting 5' ends of cDNAs Martyn White
Migration of Chromosomal vs. plasmid DNA Paul N Hengen
Ligation of single base extensions David Nunn
Your choice of Reverse Transcriptase??? Victor V. Levenson
pSV2-dhfr USA::RGB12955
PCR of a long genomic fragment... HELP? PWATER at HIPPO.OCI.UTORONTO.CA
lambda gt10 vs gt11 Daniel Meier
Southerns/nylon membranes Roy Navarre
PCR of a long genomic fragment... HELP? Basavaraju Shankarappa
Your choice of Reverse Transcriptase??? Daniel Meier
Biotech Job in Singapore Wong Wui Ming
Cleaning electroporation cuvettes chai_z at wehi.edu.au
precipitation of oligos Anand K. Bachhawat
Your choice of Reverse Transcriptase??? Cornelius Krasel
lambda gt10 vs gt11 Andre Hamel
precipitation of oligos Andre Hamel
precipitation of oligos the End
ICGEB Course on Preparation, Analysis and Applications of Synthetic Oligonucleotides. FVEGA at CINVESMX.BITNET
Avian sex-determintion Dennis J. Templeton
Gt10 and Gt11 J Preiss--Seq Anal
BIOSCI/bionet Frequently Asked Questions David Kristofferson
oligo purification Jason E Stewart
lambda gt10 vs gt11 Morris F. Manolson
Autoclave electroporation cuvettes? Michael Holloway
Chromaffin cell/Story Landis paper question? Michael Holloway
M13 Banking!? GIETZ at bldghsc.lan1.umanitoba.ca
Software wanted for densitometry Francois Baneyx
Autoclave electroporation cuvettes? Terese M Barta-1
DNA for RAPD from Brassica WOCHNIAP at IBM3090.BHAM.AC.UK
Your choice of Reverse Transcriptase??? Steven Brenner
DNA for RAPD from Brassica Thomas Mitchell-Olds
etbr disposal michael coyne
Your choice of Reverse Transcriptase??? Jun Ishikawa
Southerns/nylon membranes Jun Ishikawa
precipitation of oligos chai_z at wehi.edu.au
precipitation of oligos chai_z at wehi.edu.au
PCR of a long genomic fragment... HELP? Michael Finney
Death of a lambda library Tae Shin
Looking for library Ladislav Burysek
histological sections of insects Robert Huber
oligo purification Ed Rybicki
M13 sequencing templates from XL1Blue Rosemary T. Hoffman
Quick question on chemical name Michael van Waes
DidYouKnow... Patricia Keenan
Software wanted for densitometry Robert Horton
DidYouKnow... (Ending the thread?) John Nash
M13 sequencing templates from XL1Blue Rosemary T. Hoffman
DidYouKnow... Thomas Nguyen
DidYouKnow... Patricia Keenan
DidYouKnow... Ben Yau
Death of a lambda library Russell A. Maurer
DidYouKnow... Kees van den Doel
precipitation of oligos Ernest Retzel 1535 49118
DidYouKnow... R Clark x4971
M13 sequencing templates from XL1Blue Bruce Roe
5' end-labelling with S-35-ATP tlyoung at kean.ucs.mun.ca
DidYouKnow... Eugene Tanke
Broad host-range clone banks David Nunn
DidYouKnow... Michael van Waes
Mouse Monoclonal Antibody Subtypes Mark Bradley
Mouse Monoclonal Antibody Subtypes Corinne L Reimer
NovaTope Epitope Mapping Torban Bennett
Help- monoclonal antibodies against mouse antibody variable region Angelo Gunasekera
DidYouKnow... (Ending the thread?) David Kristofferson
Chemiluminescent Kits Dubear Kroening
Northern sensitivity Helmut Dotzlaw
Your choice of Reverse Transcriptase??? Helmut Dotzlaw
How to check if DNA is transfered Charles Rivers
DidYouKnow... Anna K. Haynes
How to check if DNA is transfered Klaus.Matthaei at anu.edu.au
DidYouKnow... Daniel L Snider
Genomic DNA Isolation - Method Required michael_m at vcp1.vcp.monash.edu.au
5' labelling with S-35 skspoidn at reading.ac.uk
Software wanted for densitometry Fourie Joubert
jcfvuy hgvcgh
Insert instability braam at DINGO.RICE.EDU
Results of Bibliography Software Survey John Alsobrook
Hi, Mr. K.R. Raj
S17-1, anybody can provide it?? Tao Tao
sheared DNA/nuclease inactivation John H McDonald
Insert instability Jim Gale
testing the local bionet Bruce
ubiqinone-1 Prince of Wales Endocrinology Unit
Insert instability the End
Death of a lambda library Eamonn Gormley
Insert instability BENNY SHOMER 9238
How to check if DNA is transfered Hiljar Sibul
Insert instability GIETZ at bldghsc.lan1.umanitoba.ca
Source for 14C-cellulose vernon
Hi, Mark Bradley
MAAPing suggestions? Dr. J. Lorenzen
Information about pR61 wanted Paul Walden
Please teach me subtraction of mRNA. alvarez at mbcf.stjude.org
Transcription Factor for pol III gene Lakshman Ramamurthy
Empigen BB - a query mhaddara at engr
5' end-labelling with S-35-ATP Brian Andersen
Death of a lambda library James R. Mensch Jr.
Expression of fusion proteins in E. coli J Preiss--Seq Anal
How to check if DNA is transfered Paul N Hengen
Expression of fusion proteins in E. coli the End
Software wanted for densitometry Francois Baneyx
ubiqinone-1 marder at agri.huji.ac.il
Has anyone isolated mRNA from rough ER? Michiaki Masuda
precipitation of oligos Cornelius Krasel
Weight on southern stacks J Preiss--Seq Anal
quantifying RT-PCR Marci Swede
Cell flow assay for luciferase Michael Cantrell
Protein Data Bank Ann Kleinschmidt
Agarose purified DNA michael at uwovax.uwo.ca
Insert instability Martin Kennedy
How to check if DNA is transfered VANNESTEJ%RUASV1 at ruakura.cri.nz
magnetic beads/mRNA labelling URA1335 at ARTHUR.CITI2.FR
S17-1, anybody can provide it?? Thon de Boer
Ligation Help Requested E85J000
Marijuana Info, alstublieft WILLIAM JAMES STEWART
Ligation Help Requested Ashok Aiyar
Protein Data Bank Dan Davison
Protein Data Bank Dan Jacobson
Expr of fusion proteins in E. coli GIETZ at bldghsc.lan1.umanitoba.ca
about gopher "7m] ..." (was Re:Protein Data Bank) Francis Ouellette
about gopher "7m] ..." (was Re:Protein Data Bank) Dan Jacobson
Ligation Help Requested GIETZ at bldghsc.lan1.umanitoba.ca
Marijuana Info, alstublieft Bwana Greg Nessinger
about gopher "7m] ..." (was Re:Protein Data Bank) Francis Ouellette
Ligation Help Requested Zophonias Oddur Jonsson
fluorimeters ??? Michelle Waycott
Biotech Job in Singapore Wong Wui Ming
luminescent westerns braam at DINGO.RICE.EDU
precipitation of oligos rupp at embl-heidelberg.de
Southerns/nylon membranes Mike Morris
Ligation Help Requested Fourie Joubert
Marijuana Info, alstublieft Stuart W. Marlatt
RNA extraction and purification from faeces! Mr D J Plows
precipitation of oligos DAVID F. BISHOP
Identification of Transcription Factor DNA binding sites - Protocols? Anthony D. Shearing
luminescent westerns Patricia Foster
Ligation Help Requested Patricia Foster
Identification of Transcription Factor DNA binding sites - Protocols? Mark Guiltinan
For human endotherial cell line Kiichi Hirota
codon preferences in plants (S. oleracea) Lars Snogerup
codon preferences in plants (S. oleracea) Lars Snogerup
Source of radiolabelled polysaccharides vernon
summary:chemiluminscent westerns braam at DINGO.RICE.EDU
codon preferences in plants (S. oleracea) Basavaraju Shankarappa
Genomic DNA Isolation - Method Required Andre Hamel
Clontech strikes again 8-( Roger Wiegand
spin-columns (homemade) Bill Melchior
fluorimeters ??? the End
magnetic beads/mRNA labelling Andre Hamel
Degenerate primers for PCR cloning BOB RUTLEDGE
Ligation Help Requested Andre Hamel
Clontech strikes again 8-( Michael Finney
Peptide-amino acid separation Darrick Carter
luminescent westerns Euan R. Taylor
Urchin Oocyte cDNA expression library (query) Christopher Kashina Patil
Clontech strikes again 8-( Francis Ouellette
peptide-amino acid separation RPSCOTT
Request Expresion Vectors mreina at cnbvx3.cnb.uam.es
"FAQ" -electroporation cuvettes, cleaning of. rpgrant at molbiol.ox.ac.uk
Image Analysis System RPSCOTT
unsubscribe David Bradbury
Periplasmic Preps Smoke me a kipper. I'll be back for breakfast!
precipitation of oligos rupp at embl-heidelberg.de
spin-columns (homemade) Basavaraju Shankarappa
"FAQ" -electroporation cuvettes, IGNORE!! rpgrant at molbiol.ox.ac.uk
purging Agro from transformants (withering heights?) J Preiss--Seq Anal
staining western blots for oligosaccharide David A Rintoul
fusion/histidine/metal binding Johan de Boer
PCR and Agarase neale at mbcf.stjude.org
Protein Data Bank Paul Wakenight
Miniflourometer (SUMMARY) neale at mbcf.stjude.org

Last message date: Wed Mar 31 19:07:02 EST 1993
Archived on: Sun Mar 6 19:48:57 EST 2005

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