quantitative westerns

Michael Holland MHOLLAND at CBR.DWE.CSIRO.AU
Mon May 3 17:28:34 EST 1993


The easiest thing may be to use a second antibody linked to peroxidse (or
anything else) and cut out your spot and dissolve in DMSO then count.
Alternatively if the isotope is a good beta emitter simply transfer and put
it down with some Saran wrap to stop the nitrocellulose sticking to your film.
Both work fine.
Good luck,
Mike "Kangaroo" Holland




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